Thursday, July 13, 2017

Xen implant demonstration






On 12th July 2017, had the opportunity to visit Dr Peter Kong Specialist Eye Center in Kota Kinabalu and witness the first Xen implantation in Sabah state of Malaysia. The first patient was a lady with primary angle closure glaucoma in whom a Xen implantation alone was done. The other, another female patient had an open angle glaucoma, in whom a phacoemulsification as well as Xen implantation was done.




Observing the technique from close, it seems like there is a learning curve to this procedure, though not a very steep one. Two things noticed were: (i) the insertor can be made more user friendly and (ii) the company should find an appropriate blade to put the insertor in the anterior chamber so that the insertor and anterior chamber depth remain stable during the procedure.

Sunday, July 9, 2017

Retinal Nerve Fiber Layer Thickness (RNFLT) assessment 


This is a primer regarding: "Retinal Nerve Fiber Layer Thickness (RNFLT)  assessment using the CIRRUS Optical coherence tomography (OCT) machine".



The Cirrus RNFL map represents a 6 x 6 mm cube of A-scan data centered over the optic nerve in which a 3.4 mm diameter circle of RNFL data is extracted to create what is referred to as the TSNIT map (temporal, superior, nasal, inferior, temporal).  It is displayed as a false color scale with the thickness values referenced to a normative database. The TSNIT map displays RNFL thickness values by quadrants and clock hours, and the RNFL peaks give a sense of the anatomic distribution of nerve fiber axons represented by the superior and inferior bundles that emanate from the optic nerve.


SD-OCT measurements are compared against an age-matched normative database. The normative database for the Cirrus SD-OCT consisted of 284 healthy individuals with an age range between 18 and 84 years (mean of 46.5 years). Ethnically, 43% were Caucasian, 24% were Asians, 18% were African American, 12% were Hispanic, 1% were Indian, and 6% were of mixed ethnicity. The refractive error ranged from -12.00 D to +8.00 D.  Due to this relatively small normative database and wide variation of distribution of RNFL, many results obtained by SD-OCT may be flagged as abnormal statistically in patients who are not represented in the database and thus not necessarily representing real disease. Clinicians should use caution to avoid overtreating “red disease” in these situations.


The Cirrus normative comparison for ONH parameters is based on the patient’s age and disc size; while, for the RNFL is based on patient’s age. For a particular age and disc size the patient is expected to have rim volume, C/D ratio etc within certain ranges. Those parameters are shaded red, yellow, green and white based on how they compare with normal ranges. When no normative data is available for comparison, the parameters are shaded grey. This applies to disc areas <1.3 mm2 or >2.5 mm2, since the database has insufficient number of subjects with the disc areas of these sizes.


Average RNFL thickness indicates a patient's overall RNFL health. The mean value for RNFL thickness in the general population is 92.9 +/- 9.4 microns. Typically, a normal, non-glaucomatous eye has an RNFL thickness of 80 microns or greater. An eye with an average RNFL thickness of 70 to 79 is suspicious for glaucoma. An average thickness of 60 to 69 is seen in less than 5% of the normal population and implies glaucoma.

Based on a longitudinal study, the age-related rate of reduction in RNFL thickness has been estimated to be -0.52 µm/year, -1.35 µm/year, and -1.25 µm/year for average, superior, and inferior RNFL respectively.

The best quality scans have signal strength greater than 8 (minimum acceptable scan > 6).


(A). METHOD:

                                 i.OCT images can be acquired through a 3mm pupil in the absence of media opacities.

                                ii.However, a dilated pupil makes the procedure easier and reduces acquisition errors.

                           iii. The patient can be asked to blink a few times before image acquisition is started.

                                  iv. A lubricant eyedrop can be instilled in case of dry eyes.

                                 v.The patient is asked to look into an internal fixation target. (Green star-like in Cirrus)

                                  vi.A circular or rarely a linear image is then acquired. 


(B). OCT REPORT PRINT-OUT: The Cirrus report shows assessment of the RNFL and ONH of both eyes based on the 6mmx6mm cube captured by the Optic Disc Cube 200x200 scan. Some models of the OCT can display optic disc modules including parameters such as rim area, disc area, average- and vertical- C/D ratio.
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1.       KEY DATA: This forms the first part of information for the printout and consists of the date and time of test; registration number, age, sex and date of birth of the patient; technician, and the “signal strength”, which should be above 8.
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2.       RNFLT MAP: Topographic map of RNFL thickness in an hourglass shape of yellow and red colors is typical of normal eyes. The color scale in microns on the left of the image for reference is also provided. Warm colors (red, yellow) represent thicker areas, while cooler colors (blue, green) represent thinner areas.
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3.       RNFLT DEVIATION MAP: It depicts the map of RNFL deviation from normal values overlaid on an en face fundus image. It also shows the machine-derived boundary of the cup and the disc and also the calculation circle placement for the RNFL. It depicts the deviation from the normative database in the form of color-coded superpixels, utilizing only yellow and red colors. Green color is not used since most of the superpixels would be green in a normal individual and would be present over most of the image, obscuring the underlying fundus image. Thus, any region which is not red or yellow indicates it is within normal limits. This map gives a gross clue regarding the cup-disc ratio and position of the vessels in the cup. The RNFL deviation map is useful to discuss the condition with the patient.

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4.       QUADRANT AND CLOCK-HOUR RNFLT: A display of the average RNFLT along the whole calculation circle is present on the top. Quadrant and clock hour averages are given below and color coded in the same scales as rest of the report (based on their P value with respect to deviation from age-matched data in the normative database of the OCT machine). They specify the location of the pathology quadrant and clock hour wise. 



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5.       RNFL NORMATIVE DATABASE: In the protocol, normative database is visualized using a white-green-yellow-red color code. Color coding indicates the particular position of the A-scan in the graph, the quadrant mean values and the clock-position in the circular graphs and right and left columns of the table of data.  The patient’s RNFL is marked by a black (continuous [right eye] or dashed line [left eye]). If the line dips into the red area, it indicates thinning of the RNFL.

In an age-matched normal population, the percentiles regard each specific measurement of RNFL in the following way:

(i) The thickest 5% of measurements fall in the white area (White indicates >95%).

(ii)90% of measurements fall in the green area (Green falls between 5% and <95%).

(iii)The thinnest 5% of measurements fall in or below the yellow area (Yellow between 1% and <5%, indicating “suspect” area).

(iv)The thinnest 1% of measurements fall in the red area. Measurements in red area are considered outside normal limits (Red area falls in <1%).



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6.       SYMMETRY: It indicates the extent of symmetry of the RNFLT in the TSNIT quadrants between the 2 eyes.
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7.       RNFL-TSNIT THICKNESS GRAPH: This shows the plot of RNFLT on the Y-axis (vertical) and the retinal quadrants on the X-axis (horizontal). Normally, this has a “double-hump” appearance due to the thicker RNFL in the superior and inferior quadrants. 
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8.       EXTRACTED RNFL TOMOGRAMS: They display the reflectivity of the RNFL. They are not of much clinical significance as far as decision making is considered.


Saturday, July 8, 2017

OPTICAL COHERENCE TOMOGRAPHY

GENERAL CONSIDERATIONS


  • Structural changes in glaucoma may precede functional changes seen on visual field (VF) examinations.
  • Upto 20% of the retinal ganglion cells (RGC) could be damaged before any changes occur in VFs.
  • Structural changes in glaucoma have been observed in the retinal nerve fiber layer (RNFL), optic nerve head (ONH) and other layers of the macula.
  • A number of investigations are available to study these structural changes. These include:
  1. Optical coherence tomography (OCT)
  2. Confocal scanning laser ophthalmoscopy (e.g. Heidelberg Retinal Tomography)
  3. Scanning laser Polarimetry (e.g. GDx, Nerve Fiber Analyzer)
  • OCT remains the most commonly used technique.
  • It is more efficient compared to HRT and GDx in the detection of localized RNFL defects and changes occurring in the peripapillary area.
  • OCT provides real-time qualitative (morphology & reflectivity) and quantitative (thickness, mapping and volume) analyses of the examined tissues.
  • OCT can be used for evaluation of the corneal thickness, anterior segment (angle) as well as the posterior segment.
  • OCT uses low coherence infrared light (830nm) and is based on the principle of Michelson Interferometry. The long wavelength of the light used permits it to penetrate deep to the target tissue.
  • Light in the OCT is broken into 2 arms. A sample arm (which has the item of interest) and a reference arm (usually a mirror). The combination of reflected light from the sample arm and reference light from the reference arm gives rise to an interference pattern, but only if light from both arms has traveled the “same” optical distance. (Same meaning a difference of less than a coherence length.)
  • OCT has evolved from time-domain (TD-OCT) to spectral-domain (SD-OCT).
  • In TD-OCT the path length of the reference arm is varied in time (the reference mirror is translated longitudinally). The repeated movement of the mirror permits repeated scans at different depths. A single coherent measurement focused at a single structure at a given depth is called an “A-scan”. The maximum scan rate can be 17,000 A-scans/second. This provides a 1-dimensional measurement, which is converted to a 2-dimensional image (“B-scan”). Thus, different tissue layers can be acquired in the point of focus. The section of tissue thus obtained is called a “Tomogram”.( The word tomography is derived from ancient Greek τόμος tomos, "slice, section" and γράφω graphō, "to write"). The image is viewed in real time using a scale of false colors representing the degree of backscattering of the light by the tissues at different depths. A collection of parallel B-scans help to form a “3-D” data set.
  • In FD-OCT the broadband interference (also called the “interference spectrum”) is acquired with spectrally separated detectors. Due to the Fourier relation, the depth scan can be immediately calculated by the Fourier transform from the acquired spectra, without movement of the reference arm. This feature reduces image acquisition time by simultaneous analysis at different tissue depths (27,000 A-scans per second), reducing any errors due to movement and more accurate (upto 2µ). The disadvantage is that light cannot reach the retina in the presence of lens opacities and fixation must be extremely steady.
  • “OCT IMAGES ARE NOT IMAGES OF A STRUCTURE BUT A MATHEMATICAL RECONSTRUCTION BUILT ON A PHOTOGRAPH OF THE FUNDUS”
  • The structures visualized are the result of selective absorption and selective reflection by the structure or interface illuminated by the light.
  • The strength of the signal reflected by a specific tissue depends on properties like tissue reflectivity, the amount of light absorbed by the overlying tissues and the amount of reflected light which reaches the sensor after it has been further attenuated by the interposed tissue. (When the strength of the reflected signal is strong, the scanned tissue has high reflectivity and vice versa)  [When the structures are perpendicular to the ray, reflectivity is greatest and produces red images on OCT].
  • Reflectivity of tissues:
  1. The most reflective structures on OCT are: RNFL, internal limiting membrane, junction between inner and outer segments of photoreceptors, retinal pigment epithelium [RPE]-Bruch’s membrane-choriocapillaries complex. These appear “red” on OCT.
  2. The least reflective structures are: Inner- and outer-nuclear layers, ganglion cell layer and photoreceptors. They appear “black” on OCT images.
  3. Intermediate reflective structures are: Inner- and outer-plexiform layers and external limiting membrane. These appear “green” on OCT.